Table 2.
The influence of AM251 (1 µM) on the vasoconstriction to phenylephrine (Phe) and thromboxane analog U46619 and vasorelaxant effects of acetylcholine (Ach) and sodium nitroprusside (SNP) in the endothelium-intact aortas from normotensive rats: control (WKY) and URB597-treated (WKY + URB), or hypertensive rats: (SHR) and URB597-treated (SHR + URB).
| Group | WKY | WKY + URB | SHR | SHR + URB |
|---|---|---|---|---|
| Phe | (7) | (7) | (7) | (7) |
| pEC50 | 7.3 ± 0.05 | 7.2 ± 0.03 | 7.3 ± 0.06 | 7.5 ± 0.05 |
| Rmax (%) | 82.9 ± 2.9 | 82.9 ± 4.1 | 88.8 ± 4.0 | 92.5 ± 2.8 |
| Phe + AM251 | (7) | (7) | (7) | (7) |
| pEC50 | 7.2 ± 0.03 | 7.4 ± 0.10 | 7.4 ± 0.06 | 7.4 ± 0.05 |
| Rmax (%) | 91.3 ± 2.6 | 83.3 ± 7.1 | 94.5 ± 5.7 | 91.6 ± 4.2 |
| U46619 | (7) | (7) | (7) | (7) |
| pEC50 | 7.4 ± 0.10 | 7.4 ± 0.03 | 8.1 ± 0.05 *** | 7.9 ± 0.04 |
| Rmax (%) | 97.2 ± 2.9 | 106.8 ± 2.5 | 108.4 ± 3.6 | 104.7 ± 1.9 |
| U46619 + AM251 | (7) | (7) | (7) | (7) |
| pEC50 | 7.7 ± 0.04 | 7.5 ± 0.06 | 8.0 ± 0.04 | 7.8 ± 0.06 |
| Rmax (%) | 98.7 ± 4.0 | 108.5 ± 2.2 | 106.4 ± 2.6 | 107.9 ± 1.1 |
| Ach | (6) | (6) | (6) | (6) |
| pEC50 | 7.2 ± 0.03 | 6.8 ± 0.02 *** | 7.3 ± 0.09 | 7.7 ± 0.06 ∆∆∆ |
| Rmax (%) | 100.0 ± 6.7 | 96.2 ± 7.0 | 91.3 ± 4.7 | 92.6 ± 1.7 |
| SNP | (6) | (6) | (6) | (6) |
| pEC50 | 8.4 ± 0.08 | 8.1 ± 0.04 * | 7.6 ± 0.05 *** | 7.7 ± 0.06 |
| Rmax (%) | 113.0 ± 4.8 | 103.9 ± 2.0 | 110.0 ± 3.6 | 100.2 ± 6.9 |
URB597 1 mg/kg or its vehicle was injected intraperitoneally every 12 h for 14 days. Data are expressed as the mean ± SEM. The numbers of animals were shown within parentheses. Contractile and vasodilator responses are shown as a percentage of the reference response to 120 mM KCl and of the isometric contraction induced by phenylephrine (aortas: hypertensive 0.03 μM and normotensive 0.3 μM rats), respectively. * p < 0.05; ***,∆∆∆ p < 0.001 compared to the * WKY, ∆ SHR, as determined by one-way ANOVA followed by Bonferroni’s multiple comparison test and Student’s t-test for unpaired data.