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Fluorescence emission spectroscopy indicated that tyrosine 59 was not involved in cluster coordination in the C59Y BOLA3–GLRX5 complex. Fluorescence emission spectra of apo C59Y BOLA3–GLRX5 complex before (black) and after (orange) chemical reconstitution recorded upon excitation at 280 nm. In the inset, fluorescence emission difference spectrum (Δ) obtained by subtracting the spectrum of the chemically reconstituted hetero-complex from that of the apo hetero-complex.
