Table 1.
List of proteins identified in royal jelly found in: CRJ—“crude” (non-enriched with ProteoMinerTM technique) water extracts of royal jelly; F1, F2, F3, F4—separate fractions obtained from ProteoMinerTM elution with 1 M sodium chloride, 20 mM HEPES, pH 7.4 (F1), 200 mM glycine, pH 2.4 (F2), 60% ethylene glycol in water (F3), and 33.3% 2-propanol, 16.7% acetonitrile (ACN), 0.1% trifluoroacetic acid (TFA) (F4); MW—molecular weight; pI—isoelectric point. Fractions, in which proteins were detected, marked with “x”.
| Accession | Protein Name | Function | MW [kDa] | pI | CRJ | F1 | F2 | F3 | F4 |
|---|---|---|---|---|---|---|---|---|---|
| gi|110749126 | Predicted: glucose dehydrogenase [acceptor] isoform 3 [Apis mellifera] | Enzyme | 70.1 | 6.7 | x | ||||
| gi|110751029 | Predicted: e3 ubiquitin-protein ligase IAP-3-like [Apis mellifera] | Enzyme | 43.2 | 6.4 | x | ||||
| gi|110756431 | Predicted: hypothetical protein LOC725074 [Apis mellifera] | 8.3 | 10.2 | x | |||||
| gi|110758964 | Predicted: regucalcin-like [Apis mellifera] | Binding | 10.2 | 9.4 | x | x | |||
| gi|110763647 | Predicted: hypothetical protein LOC726323 [Apis mellifera] | 18.5 | 7.9 | x | |||||
| gi|13184963 | defensin [Apis mellifera] | Defense | 6.3 | 6.5 | x | ||||
| gi|166795901 | apolipophorin-III-like protein precursor [Apis mellifera] | Binding | 21.3 | 5.4 | x | x | x | x | x |
| gi|202078658 | defensin [Apis cerana cerana] | Defense | 10.6 | 7.8 | x | ||||
| gi|202078660 | defensin [Apis cerana cerana] | Defense | 10.7 | 7.8 | x | ||||
| gi|254548151 | defensin precursor [Apis cerana] | Defense | 8.7 | 6.4 | x | x | x | x | x |
| gi|254548155 | defensin precursor [Apis mellifera] | Defense | 8.8 | 5.9 | x | x | x | x | x |
| gi|254910938 | defensin-1 preproprotein [Apis mellifera] | Defense | 10.7 | 6.4 | x | ||||
| gi|258678306 | MRJP9 [Apis cerana] | Honeybee nutrition and development | 19.4 | 9.2 | x | ||||
| gi|258678310 | MRJP5 [Apis dorsata] | Honeybee nutrition and development | 21.3 | 4.7 | x | ||||
| gi|258678314 | MRJP6 [Apis florea] | Honeybee nutrition and development | 21.5 | 4.4 | x | x | |||
| gi|258678316 | MRJP9 [Apis florea] | Honeybee nutrition and development | 21.0 | 5.5 | x | x | x | x | x |
| gi|283105164 | alpha-glucosidase III [Apis dorsata] | Enzyme | 65.5 | 5.0 | x | x | |||
| gi|284182838 | major royal jelly protein 4 [Apis mellifera] | Honeybee nutrition and development | 53.0 | 5.9 | x | x | x | x | |
| gi|284812514 | MRJP5 [Apis mellifera] | Honeybee nutrition and development | 70.1 | 6.1 | x | x | x | x | x |
| gi|288872651 | major royal jelly protein [Apis mellifera] | Honeybee nutrition and development | 61.6 | 6.7 | x | x | x | x | x |
| gi|28972896 | major royal jelly protein-like protein [Apis dorsata] | Honeybee nutrition and development | 9.2 | 9.8 | x | x | x | x | x |
| gi|328775853 | Predicted: DNA replication licensing factor MCM4-like [Apis mellifera] | Binding | 80.7 | 6.9 | x | ||||
| gi|328777366 | Predicted: hypothetical protein LOC100577348 [Apis mellifera] | 61.1 | 10.5 | x | |||||
| gi|328779534 | Predicted: hypothetical protein LOC552041 [Apis mellifera] | 79.3 | 4.2 | x | |||||
| gi|328782858 | Predicted: hypothetical protein LOC410515 [Apis mellifera] | 190.2 | 6.4 | x | |||||
| gi|328783362 | Predicted: hypothetical protein LOC725249 [Apis mellifera] | 6.3 | 4.9 | x | |||||
| gi|328783471 | Predicted: hypothetical protein LOC725114 isoform 1 [Apis mellifera] | 10.1 | 5.7 | x | |||||
| gi|328784821 | Predicted: hypothetical protein LOC100577210 [Apis mellifera] | 11.6 | 5.9 | x | x | x | x | ||
| gi|328789019 | Predicted: protein SERAC1-like [Apis mellifera] | Remodeling | 87.5 | 9.2 | x | ||||
| gi|328790726 | Predicted: venom acid phosphatase Acph-1-like [Apis mellifera] | Enzyme | 42.6 | 8.5 | x | ||||
| gi|328792767 | Predicted: hypothetical protein LOC724993 [Apis mellifera] | 43.8 | 5.4 | x | |||||
| gi|328793775 | Predicted: s-adenosylmethionine decarboxylase proenzyme-like, partial [Apis mellifera] | Enzyme | 32.1 | 4.6 | x | ||||
| gi|328794347 | Predicted: major royal jelly protein 3-like, partial [Apis mellifera] | Honeybee nutrition and development | 42.7 | 6.5 | x | x | |||
| gi|33358394 | major royal jelly protein MRJP1 [Apis cerana cerana] | Honeybee nutrition and development | 49.0 | 5.4 | x | x | x | x | x |
| gi|380011960 | Predicted: slit homolog 2 protein-like [Apis florea] | Binding | 155.3 | 6.0 | x | ||||
| gi|380012917 | Predicted: uncharacterized protein LOC100870850 [Apis florea] | 18.9 | 7.2 | x | |||||
| gi|380013532 | Predicted: Low Quality Protein: clathrin heavy chain-like [Apis florea] | Binding | 187.7 | 5.6 | x | ||||
| gi|380016522 | Predicted: probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2-like [Apis florea] |
Enzyme | 39.9 | 9.6 | x | ||||
| gi|380017034 | Predicted: glucosylceramidase-like [Apis florea] | Enzyme | 60.8 | 5.5 | x | x | |||
| gi|380019073 | Predicted: lysozyme 1-like isoform 2 [Apis florea] | Enzyme | 13.8 | 4.6 | x | x | x | ||
| gi|380020436 | Predicted: regucalcin-like [Apis florea] | Binding | 37.7 | 5.3 | x | x | x | x | |
| gi|380022658 | Predicted: major royal jelly protein 3-like [Apis florea] | Honeybee nutrition and development | 62.7 | 7.8 | x | x | |||
| gi|380022660 | Predicted: major royal jelly protein 4-like [Apis florea] | Honeybee nutrition and development | 56.1 | 6.1 | x | ||||
| gi|380022665 | Predicted: major royal jelly protein 1-like [Apis florea] | Honeybee nutrition and development | 43.9 | 5.3 | x | x | x | x | x |
| gi|380022667 | Predicted: major royal jelly protein 2-like [Apis florea] | Honeybee nutrition and development | 49.1 | 5.7 | x | x | |||
| gi|380022669 | Predicted: major royal jelly protein 2-like [Apis florea] | Honeybee nutrition and development | 49.3 | 6.0 | x | x | x | x | x |
| gi|380022673 | Predicted: major royal jelly protein 5-like [Apis florea] | Honeybee nutrition and development | 34.2 | 4.7 | x | ||||
| gi|380022681 | Predicted: major royal jelly protein 5-like isoform 2 [Apis florea] | Honeybee nutrition and development | 47.5 | 9.2 | x | ||||
| gi|380023404 | Predicted: uncharacterized protein LOC100869599 [Apis florea] | 18.7 | 9.5 | x | x | x | |||
| gi|380024584 | Predicted: chymotrypsin inhibitor-like [Apis florea] | Enzyme inhibitor | 5.6 | 10.0 | x | ||||
| gi|380024588 | Predicted: chymotrypsin inhibitor-like [Apis florea] | Enzyme inhibitor | 8.0 | 5.0 | x | ||||
| gi|380025248 | Predicted: alkylated DNA repair protein alkB homolog 8-like [Apis florea] | Enzyme | 68.2 | 9.3 | x | x | |||
| gi|380025500 | Predicted: venom acid phosphatase Acph-1-like [Apis florea] | Enzyme | 39.9 | 4.9 | x | ||||
| gi|380025661 | Predicted: glucose dehydrogenase [acceptor]-like [Apis florea] | Enzyme | 67.9 | 6.4 | x | x | x | x | |
| gi|380026601 | Predicted: uncharacterized protein LOC100863702 [Apis florea] | 9.9 | 5.0 | x | x | ||||
| gi|380027252 | Predicted: uncharacterized protein LOC100864410 [Apis florea] | 33.4 | 9.7 | x | |||||
| gi|380028593 | Predicted: Low Quality Protein: delta-1-pyrroline-5-carboxylate synthase-like [Apis florea] |
Enzyme | 84.9 | 9.1 | x | ||||
| gi|40218299 | major royal jelly protein MRJP5 [Apis cerana cerana] | Honeybee nutrition and development | 70.5 | 8.8 | x | ||||
| gi|40218301 | major royal jelly protein MRJP2 [Apis cerana cerana] | Honeybee nutrition and development | 53.0 | 9.1 | x | x | x | ||
| gi|40557703 | major royal jelly protein MRJP1 precursor [Apis cerana] | Honeybee nutrition and development | 48.9 | 5.4 | x | x | x | x | x |
| gi|40557705 | major royal jelly protein MRJP2 precursor [Apis cerana] | Honeybee nutrition and development | 52.5 | 8.9 | x | x | x | x | x |
| gi|42601246 | major royal jelly protein MRJP5 precursor [Apis cerana] | Honeybee nutrition and development | 68.2 | 9.3 | x | x | |||
| gi|46358503 | major royal jelly protein 2 [Apis cerana] | Honeybee nutrition and development | 52.4 | 8.9 | x | x | x | x | x |
| gi|48094573 | Predicted: hypothetical protein LOC408608 [Apis mellifera] | 19.4 | 7.5 | x | x | ||||
| gi|48101366 | Predicted: venom serine protease 34 [Apis mellifera] | Enzyme | 44.6 | 5.9 | x | ||||
| gi|562090 | defensin precursor [Apis mellifera] | Defense | 10.7 | 6.4 | x | x | |||
| gi|56422035 | major royal jelly protein 3 [Apis mellifera carnica] | Honeybee nutrition and development | 65.7 | 7.1 | x | x | x | x | x |
| gi|56422037 | major royal jelly protein 3 [Apis cerana] | Honeybee nutrition and development | 69.2 | 9.3 | x | x | x | ||
| gi|56422041 | major royal jelly protein 3 [Apis florea] | Honeybee nutrition and development | 59.3 | 6.4 | x | ||||
| gi|57546160 | major rojal jelly protein 7 [Apis cerana] | Honeybee nutrition and development | 24.9 | 5.5 | x | x | x | x | x |
| gi|58585090 | glucose oxidase [Apis mellifera] | Enzyme | 67.9 | 6.5 | x | x | |||
| gi|58585098 | major royal jelly protein 1 precursor [Apis mellifera] | Honeybee nutrition and development | 48.9 | 5.0 | x | x | x | x | x |
| gi|58585108 | major royal jelly protein 2 precursor [Apis mellifera] | Honeybee nutrition and development | 51.0 | 7.0 | x | x | x | x | x |
| gi|58585138 | major royal jelly protein 5 precursor [Apis mellifera] | Honeybee nutrition and development | 70.2 | 5.9 | x | ||||
| gi|58585142 | major royal jelly protein 3 precursor [Apis mellifera] | Honeybee nutrition and development | 61.6 | 6.5 | x | x | x | ||
| gi|58585164 | alpha-glucosidase precursor [Apis mellifera] | Enzyme | 65.5 | 4.9 | x | ||||
| gi|58585170 | major royal jelly protein 4 precursor [Apis mellifera] | Honeybee nutrition and development | 52.9 | 5.9 | x | x | x | x | x |
| gi|58585188 | major royal jelly protein 6 precursor [Apis mellifera] | Honeybee nutrition and development | 49.8 | 5.9 | x | x | x | x | x |
| gi|60115688 | icarapin-like precursor [Apis mellifera] | Venom carbohydrate-rich protein | 24.8 | 4.4 | x | x | x | x | |
| gi|62198227 | major royal jelly protein 7 precursor [Apis mellifera] | Honeybee nutrition and development | 50.5 | 4.8 | x | x | x | x | x |
| gi|66504790 | Predicted: integrator complex subunit 7-like isoform 1 [Apis mellifera] | snRNA processing | 105.9 | 9.5 | x | ||||
| gi|66511554 | Predicted: glucosylceramidase-like isoform 1 [Apis mellifera] | Enzyme | 59.3 | 5.2 | x | x | x | x | x |
| gi|66564326 | Predicted: plasma glutamate carboxypeptidase-like isoform 1 [Apis mellifera] | Enzyme | 52.9 | 5.1 | x | ||||
| gi|66565246 | Predicted: lysozyme isoform 1 [Apis mellifera] | Enzyme | 17.1 | 4.6 | x | x | |||
| gi|76496465 | major royal jelly protein 3 [Apis dorsata] | Honeybee nutrition and development | 66.9 | 6.6 | x | x | x | x | x |
| gi|94471624 | icarapin variant 2 precursor [Apis mellifera] | Venom carbohydrate-rich protein | 19.6 | 4.2 | x |