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. 2021 May 1;13(9):2182. doi: 10.3390/cancers13092182

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Gene expression analysis after transient silencing of PDE4D gene. (A) Heatmap representation of cancer-related genes analysed by TaqMan OpenArray that were up-regulated (left panel) or down-regulated (right panel) in HepG2 cells silenced or not silenced for PDE4D. This image was generated using online tools provided by CIMminer (http://discover.nci.nih.gov/cimminer/home.do, accessed on 23 February 2021). IGF2 gene expression analysis by qRT-PCR in HepG2 (B), Hep3B (C), and Huh7 (D) cells 48 h after transient silencing. Data are the mean ± SD of three independent experiments. Student t test. ** p < 0.01; *** p < 0.001 versus Scr-siRNA.