Figure 6.

The effect of Gebr-7b on cell proliferation, cells cycle and apoptosis in HCC cells. Cell proliferation assay performed by DELFIA assay in HepG2 (A) and Huh7 (B) cells. Value expressed in EU counts. Cell cycle profiles measured by propidium iodide staining and flow cytometry analysis in HepG2 (C) and Huh7 (D) cells. Flow cytometry evaluation of apoptosis using Annexin V-FITC/PI staining in HepG2 (E) and Huh7 (F) cells. Data are the mean ± SD of three independent experiments. Student t test. * p < 0.05; ** p < 0.01; *** p < 0.001 versus vehicle.