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. 2021 May 8;13(9):2255. doi: 10.3390/cancers13092255

Figure 2.

Figure 2

MM cells producing PTX3 are less pro-angiogenic. (A) KMS-11 PTX3 cells embedded in alginate pellets containing (KMS-11 PTX3+DOXA) or not (KMS-11 PTX3-DOXA) doxycycline were grafted onto the top of the chick embryo CAM at day 11 of development. PBS (Vehicle) or DOXA were used as control. At day 14, for each embryo, the number of newly formed blood vessels converging towards the implant were quantified. n = 15 embryo/group. Representative images of CAMs at day 14 are reported. White dashed lines show the alginate pellet implants. (B) GFP-expressing MM.1S PTX3 cells in vitro induced (MM.1S PTX3+DOXA) or not (MM.1S PTX3-DOXA) with DOXA were grafted into the perivitelline space of 48 hpf Tg (fli1:egfp) zebrafish embryos. Twenty-four hours after engraftment, for each embryo, the cumulative length of sprouts deriving from subintestinal vein vessels was quantified. In the magnified images, the tumor mass is highlighted in red and the vessel sprouts are indicated with arrows. n = 30 embryo/group. In box and whiskers graphs, boxes extend from the 25th to the 75th percentiles, lines indicate the median values and whiskers indicate the range of values. * p < 0.05, # p < 0.001.