Figure 3.

PTX3 released by MM cells reduces tumor vascularization and growth. KMS-11 PTX3 cells were subcutaneously engrafted in NOD/SCID mice receiving (+DOXA) or not (-DOXA) doxycycline in the drinking water. (A–C) Histological analyses of tumor sections eighteen days after tumor engraftment. Before sacrifice, mice were injected i.v. with sulfobiotin in order to label the whole functional vascular network. Sulfobiotin and phospho-HH3 positive area were quantified by ImageJ software. Scale bar A, B: 50 μm; scale bar C: 100 μm. (D) Left panel: Tumor volumes (mean ± SEM) measured with caliper up to 18 days after tumor implantation. n = 8 mice/group. Right panel: Tumor weights at day 18 post-implantation. In box and whiskers graphs, boxes extend from the 25th to the 75th percentiles, lines indicate the median values and whiskers indicate the range of values. * p < 0.05, ** p < 0.01, # p < 0.001.