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. 2021 May 8;26(9):2779. doi: 10.3390/molecules26092779

Figure 3.

Figure 3

Identification of 2,4,6-TriHB (5) by using the high-resolution LC-ESI(−)-QTOF-MS/MS (CID of 35 eV). (A) The mass spectrum of 2,4,6-TriHB (5) (m/z 229.0491) with the MS/MS fragmentation pattern from the enzymatic reaction of GmBPS, where benzoyl-CoA (3) and malonyl-CoA (4) were the substrates. (B) The putative mass spectrum of 2,4,6-TriHB (5) (m/z 229.0503) and its fragmentation profile obtained from the bioconversion of benzoate (2) by E. coli BL21(DE3)pLysS harboring pETDuet-BadA-GmBPS.