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. 2021 May 10;22(9):5025. doi: 10.3390/ijms22095025

Figure 6.

Figure 6

DNA damage is increased in mdx CVPCs. (A) Bar graphs showing the DNA double stranded breaks in isolated CVPCs from 24 wo WT and mdx mice as illustrated with the two images on the right. The DNA damage was evaluated as the ratio of γH2AX foci number (in green) per total number of nuclei (DAPI labeled, in blue) per image. At least 20 cells were evaluated from each animal; the average from each animal was used for statistics with three animals per group (* p < 0.05). The scale bar represents 20 µm. (B) Bar graphs showing the relative fluorescence of ROS production using CellROX dye on freshly isolated CVPCs of 9 and 24 wo mice. At least three animals per group were used. The values are represented in the bar graphs for WT (open bars and black circles/dots) and mdx (black bars and grey dots). The statistical difference was calculated using two-way ANOVA.