Figure 3.

Pharmacological inhibition of SIRT2 sensitizes melanoma A375 cells to cisplatin. (A) The effects of cisplatin treatment (96 h) on the viability of control cells and those pretreated with thiomyristoyl cells were determined using the neutral red assay, mean ± standard deviation (n = 3, independent experiments). (B) Results of the colony formation assay performed on control A375 cells and those pretreated with thiomyristoyl for 72 h prior to exposure to different cisplatin concentrations. Images from a single representative experiment are shown. (C) A colony area values from the colony forming assay is shown as the mean ± standard deviation (n = 3, independent experiments). * Indicates a statistically significant difference at p < 0.05. (D) Effect of cisplatin on the accumulation of γ-H2AX in control A375 cells and those pretreated with thiomyristoyl for 72 h. Cells were treated with cisplatin for 48 h. Then, cells were harvested and protein lysates were prepared and analyzed by Western blotting.