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. 2021 May 10;13(9):1526. doi: 10.3390/polym13091526

Table 1.

Primers used for quantitative RT-PCR.

Gene Name Primer Sequence Product Size (bp)
Bovine specific GAPDH F: 5′ GCCATCACTGCCACCCAGAA 3’ 207
R: 5′ GCGGCAGGTCAGATCCACAA 3’
Bovine specific Aggrecan F: 5′ GACCAGAAGCTGTGCGAGGA 3’ 319
R: 5′ GCCAGATCATCACCACACAG 3’
Bovine specific Collagen II F: 5′ ATCAACGGTGGCTTCCACT 3’ 263
R: 5′ TTCGTGCAGCCATCCTTCAG 3’
Bovine specific Collagen IX F: 5′ GGACTCAACACGGGTCCACA 3’ 102
R: 5′ ACAGGTCCAGCAGGGCTTTG 3’
Bovine specific Collagen I F: 5′ GCGGCTACGACTTGAGCTTC 3’ 102
R: 5′ CACGGTCACGGACCACATTG 3’
Bovine specific Osteopontin F: 5′ ACTGGACTCTTCTCGCCGCC 3’ 90
R: 5′ CGGAGGCAATGCCCAAGAGGC 3’