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. 2021 May 15;21:166. doi: 10.1186/s12890-021-01521-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — As the cognate sense transcript of GATA6-AS1, GATA6 was low-expressed in LUAD cells. a GEPIA database showed the expression of GATA6 in 483 LUAD tissue samples and 347 corresponding normal tissue samples. b Expression of GATA6 in 35 LUAD tissues and non-tumor tissues was detected by RT-qPCR. c The mRNA expression and protein expression of GATA6 in LUAD cells and normal lung cells were detected by RT-qPCR and western blot. d RT-qPCR analysis was performed to reveal the overexpression efficiency of GATA6 in A549 and H1975 cells. e Western blot analysis showed the expression of GATA6 at the protein level in A549 and H1975 cells after upregulation of GATA6. f LncLocator database predicted the subcellular location of GATA6-AS1. g The main distribution of GATA6-AS1 in A549 and H1975 cells was determined utilizing the subcellular fractionation assay. *p < 0.05, **p < 0.01