Skip to main content
. 2021 May 16;2(6):100311. doi: 10.1016/j.xcrm.2021.100311

Figure 2.

Figure 2

Binding affinity of developed mAbs and the application for viral NP antigen detection

(A) Affinity measurement of selected monoclonal antibodies on the Octet RED96 instrument. Association and dissociation of each mAb to full-length NP at various concentrations (50, 25, 12.5, 6.25, 3.13, 1.56, and 0.78 nM) was evaluated using anti-mouse IgG capture (AMC) sensor (two technical replicates).

(B) Immunoblot analysis of mock or SARS-CoV-2-infected VeroE6/TMPRSS2 cell lysates (representative image of two technical replicates).

(C) Immunofluorescence analysis (representative image of two technical replicates). VeroE6/TMPRSS2 cells were infected or mock infected with SARS-CoV-2. After 24 h, cells were fixed and then stained with mAbs (hybridoma supernatant; red) and DAPI (blue).

(D) Paraffin-embedded lung biopsy specimen from a case of COVID-19 was examined for immunohistochemical detection of SARS-CoV2 using our antibody. The positive signals (arrowheads) are seen in bronchiolar epithelial cells (top), pneumocytes (middle), and endothelial cell (bottom).