Table 2.
Thermodynamic parameters estimated from thermal denaturation of Mb with various concentration ratio mixtures of PEG 400 + EG in mg mL−1 at pH 7.0, using Soret-absorption spectroscopy a.
| [PEG 400 +EG], mg mL−1 |
Δε409 | |
|---|---|---|
|
Tm (°C) |
ΔHm (kcal mol−1) |
|
| 0 + 0 | 81.5 (±0.5) |
120.20 (±0.92) |
| 0 + 50 | 78.6 (±0.60) |
120.90 (±0.93) |
| 0 + 300 | 65.26 (±0.60) |
106.30 (±0.82) |
| 50 + 0 | 78.5 (±0.61) |
98.70 (±1.4) |
| 300 + 0 | 63.2 (±0.60) |
70.10 (±0.54) |
| 50 + 50 | 77.76 (±0.23) |
85.02 (±0.70) |
| 50 + 300 | 70.69 (±0.63) |
117.27 (±0.25) |
| 300 + 50 | 73.75 (±0.5) |
90.06 (±0.17) |
a A ± with each parameter represents the mean error obtained from triplicate measurement.