Table 3.
Comparison of spectral properties of Mb in the presence of crowders (PEG 400 and EG) individually and their mixtures (PEG 400+ EG) at pH 7.0 and 25 °C a.
| Mixture of Crowders (PEG 400 + EG), mg mL−1 |
Rh (Å) |
ε409, M−1 cm−1 |
F 335 | [θ] (deg cm2 dmol−1) at Wavelength in nm |
|
|---|---|---|---|---|---|
| 222 | 272 | ||||
| No Crowder (0 + 0) |
23.46 (±0.68) |
171,645 (±285) |
29 (±1.24) |
−26,190 (±278) |
182 (±12) |
| (0 + 50) | 23.10 (±0.58) |
187,716 (±509) |
33 (±2.4) |
−26,015 (±258) |
183 (±14) |
| (0 + 300) | 22.70 (±0.60) |
169,831 (±619) |
33 (±2.0) |
−26,065 (±150) |
185 (±10) |
| (50 + 0) | 21.30 (±0.50) |
166,561 (±499) |
43 (±4.1) |
−25,119 (±204) |
159 (±7) |
| (300 + 0) | 33.8 (±0.40) |
85,776 (±152) |
120 (±5.2) |
−13,987 (±104) |
61 (±4) |
| (50 + 50) | 23.05 (±0.60) |
194,821 (±689) |
38 (±2.3) |
−27,547 (±278) |
181 (±12) |
| (50 + 300) | 22.80 (±0.70) |
183,029 (±539) |
42 (±4.4) |
−27,278 (±254) |
176 (±10) |
| (300 + 50) | 26.45 (±0.60) |
130,644 (±282) |
98 (±6.4) |
−18,908 (±121) |
105 (±6) |
a A ± with each parameter represents same as in Table 2.