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. 2021 May 2;22(6):658–672. doi: 10.1111/mpp.13052

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© 2021 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Amino acid profiling of cucumber leaf extracts. Amino acids were extracted from leaves of cucumber genotypes HS279 (downy mildew susceptible) and NIL DM4.1.3 (partially downy mildew resistant), 7 days postinoculation with Pseudoperonospora cubensis or mock treatment. Free amino acid concentrations were quantified using gas chromatography with flame ionization detection (GC‐FID). Coloured bars indicate the average amino acid concentration per amino acid (in nmol per mg dry weight) in each of the genotype × treatment combinations (n = 8 in each group). Error bars indicate standard error of the mean. Differences due to genotype, treatment, and the interaction were all found to be statistically significant (two‐way multivariate analysis of variance, p < .05). Asterisks indicate significant between‐subject differences (p < .05)