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. 2021 May 10;13(9):2284. doi: 10.3390/cancers13092284

Figure 2.

Figure 2

Effect of the combination of PLX4032 and orlistat in LM16 R cells. (A) Cells were exposed to single agents or to their combination and harvested 48 h after treatment for analysis of apoptotic response. Apoptosis was assessed by Annexin V-binding assay. Histograms represent the mean ± SEM of 3 independent experiments. * p < 0.05 by one-way ANOVA followed by Bonferroni correction. (B) Western blot analyses were carried out in exponentially growing cells. Cells were exposed to the drugs alone or in combination for 48 h and harvested for protein extraction. Samples were loaded on SDS-PAGE. Equal loading is shown by tubulin. (C) Caspase 3/7 activation evaluated in cells treated with PLX4032 alone or in combination with orlistat, by subtracting the untreated control. * p < 0.05 by one-way ANOVA followed by Bonferroni correction compared to single agents. The detected levels were significantly increased for all the treatments compared to untreated control (p < 0.05).