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. 2021 May 10;13(9):2284. doi: 10.3390/cancers13092284

Figure 3.

Figure 3

Effects of FASN inhibition on DHCR24 regulation. (A) DHCR24 levels evaluated by Western blot upon knockdown of FASN by RNA interference in LM16 at 48 h and in LM16 R cells at 72 h. Equal loading is shown by tubulin. (B) FASN and DHCR24 levels in LM16 R cells after 48 h treatment with orlistat (3 µM) and PLX4032, as single agents or in combination. Equal loading is shown by actin. (C) Expression levels of SREBF1, SREBF2, DHCR24 and FASN as determined by qRT-PCR in LM16 R cells upon treatment with orlistat (3 µM). Relative quantification (RQ) values are shown.* p < 0.05, ** p < 0.01, *** p < 0.0001 by unpaired Student’s t test compared to the vehicle that is (DMSO)-treated.