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. 2021 May 10;22(9):5048. doi: 10.3390/ijms22095048

Figure 5.

Figure 5

ThHSFA1 decreases ROS levels in T. hispida plants under salt stress. (A,B) DAB and NBT staining were used to measure H2O2 and O2•− levels. (C–F) H2O2 and O2•− content and relative POD and SOD activity levels in T. hispida plants. The transiently transformed T. hispida plants were grown on half-strength Murashige–Skoog (1/2 MS) medium supplemented with 0 (control) or 150 mM NaCl (salt) for 24 h, and leaves were collected for histochemical staining and physiological analyses. VC: empty pROKII-transformed T. hispida plants; RNAi: T. hispida plants with transient RNAi-mediated silencing of ThHSFA1; OE: T. hispida plants with transient overexpression of ThHSFA1. The error bars represent ± SD from multiple biological replicates, and the asterisks indicate significant differences between the VC and RNAi or OE plants (*, p < 0.05).