Figure 3.

E2 selectively stimulates ERβ4 and ERβ5 isoforms’ mRNA abundance in hGCs. Cells were treated with solvent vehicle (V) or 10 nM E2 for 24 h. mRNAs were extracted and mRNA levels of ERα (A) and ERβ (1, 2, 4, and 5) isoforms (B) were measured relative to that of GAPDH by RT-qPCR. E2 significantly stimulates the relative transcript levels of ERβ4 and ERβ5 isoforms (n = 49). Values are represented as means ± SEM from two or three identical wells per patient, measured in triplicate. Statistical analysis was performed by the two-side Wilcoxon signed ranks test. A.U., Arbitrary Units.