Table 1.
Primers used for quantitative real-time RT-PCR.
| Target Gene (Protein) Accession No. |
Forward Primer (Tm) Reverse Primer (Tm) |
Product Size, Amplification Efficiency |
|---|---|---|
|
ESR1 (ERα) NM_001122740 |
5’-CCA CCA ACC AGT GCA CCA TT-3’ (59.4 °C) 5’-GGT CTT TTC GTA TCC CAC CTT TC-3’ (60.6 °C) |
116 bp, 97.2% |
|
ESR2 v1 (ERβ1) NM_001437 |
5’-GTC AGG CAT GCG AGT AAC AA-3’ (57.3 °C) 5’-GGG AGC CCT CTT TGC TTT TA-3’ (57.3 °C) |
192 bp, 90.9% |
|
ESR2 v2 (ERβ2) NM_001040275 |
5’-TCT CCT CCC AGC AGC AAT CC-3’ (61.4 °C) 5’-GGT CAC TGC TCC ATC GTT GC-3’ (61.4 °C) |
162 bp, 96% |
|
ESR2 v4 (ERβ4) NM_001214902 |
5’- GTG ACC GAT GCT TTG GTT TG-3’ (57.3 °C) 5’-ATC TTT CAT TGC CCA CAT GC-3’ (55.3 °C) |
210 bp, 96% |
|
ESR2 v5 (ERβ5) DQ838583.1 |
5’-GAT GCT TTG GTT TGG GTG AT-3’ (55.3 °C) 5’-CCT CCG TGG AGC ACA TAA TC-3’ (59.4 °C) |
177 bp, 95% |
|
GAPDH (GAPDH) NM_002046.7 |
5’-TCC CTG AGC TGA ACG GGA AG-3’ (61.4 °C) 5’-GGA GGA GTG GGT GTC GCT GT-3’ (63.5 °C) |
227 bp, 98.5% |