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. Author manuscript; available in PMC: 2021 May 16.
Published in final edited form as: Neurogastroenterol Motil. 2020 Nov 10;33(4):e14030. doi: 10.1111/nmo.14030

FIGURE 6.

FIGURE 6

3D images showing hpChAT-ir cholinergic neuronal cells and nerve fiber bundles in the submucosal plexus (SMP) and myenteric plexus (MP) of human sigmoid colon after passive CLARITY technique. A, Note that hpChAT-ir cells are distributed in three subplexuses of the SMP: the inner submucosal plexus (ISP), outer submucosal plexus (OSP), and the third intermediate submucosal plexus (IMSP) between the ISP and OSP. B, A side view of Figure 6A after 90° rotation shows the three ganglion layers and connecting fiber bundles in the SMP. C, A 3D image of the MP with a single layer of ganglia and an intrinsic cholinergic nerve network consisting of primary nerve fiber strands (NFS-I) and their two-tier branches (NFS-II and III) pointed by arrows. The 360-degree views of Figure 5A and C are also shown in Video S3A and B