Figure 1. miR-139 induces IFN-β expression in prostate cancer cells. PC3, Du145 and LNCaP cells were transfected with either miR-139 or negative mimic for days 1, 2 and 3 (PC3, Du145) or days 3, 6, 8 and 12 (LNCaP). Conditioned media (CM) was collected and analyzed by Western blot probed with anti-IFN-β. Lysates were also blotted for IFN-β, and β tubulin for loading controls.
