Figure 3. miR-139 binds to RIG-1. HEK293T cells were treated with RIG-1 plasmid for 3 days. 35 μg of lysate were incubated with either biotin-labeled miR-139 mimic, biotin-labeled negative mimic, or biotinlabeled miR-139 mimic together with unlabeled miR-139 mimic followed by streptavidin Sepharose beads. Lysates were also incubated with streptavidin-sepharose beads alone. After washing, protein bound to beads were removed by boiling, then analyzed by WB for RIG-1 expression using an anti-DDK antibody. For input, 0.5 μg of lysate is also shown on WB, with β tubulin as loading control.
