Figure 6. Both miR-139 and IFN-β induce identical downstream signaling responses. (A) PC3 or Du145 cells were transfected for 3 days with either miR-139 mimic, negative mimic, IFN-β or no stimulation (Ø). WBs were performed using the specified antibodies, followed by stripping and reprobing with β-tubulin for loading control. Two alternatively spliced isoforms of STAT1 exist, the antibody used detects both of them. (B) PC3 cells were transfected for 3 days with either miR-139 mimic, negative mimic or IFN-β followed by WB with anti-LC3B or β-tubulin. (C) PC3 cells were transfected for 3 days with either miR-139 mimic, negative mimic, or miR-139 mimic together with anti-miR-139 antagomir. WB for IFN-β and RIG-1 are shown, with β-tubulin as loading control.