Table 1.
Summary of Tests Performed for the Various Experimental Cohorts
| Mouse cohort Blast Procedure 1 Procedure 2 Procedure 3 Procedure 4 Procedure 5 Procedure 6 |
|---|
| Cohort 1 1 × Sham/1 × Blast Delay fear Cond/Ext/Ret/Ren test (1 week) RT-qPCR (1 week) |
| Cohort 2 1 × Sham/1 × Blast Delay fear Cond/Ext/Ret/Ren test (2 weeks) RT-qPCR (2 weeks) |
| Cohort 3 1 × Sham/1 × Blast Delay fear Cond/Ext/Ret/Ren test (4 weeks) RT-qPCR (4 weeks) |
| Cohort 4 1 × Sham/1 × Blast Delay fear Cond/Ext/Ret/Ren test (8 weeks) RT-qPCR (8 weeks) |
| Cohorts 6 and 8 4 × Sham/4 × Blast Delay fear Cond/Ext/Ret/Ren test (3 days) |
| Cohorts 7, 9, and 12 4 × Sham/4 × Blast Delay fear Cond/Ext/Ret/Ren test (2 weeks) |
| Cohorts 5, 10, and 11 4 × Sham/4 × Blast Delay fear Cond/Ext/Ret/Ren test (8 weeks) |
| Cohort 13 4 × Sham/4 × Blast Trace fear cond test (3 days) Trace fear cond test (3 days) |
| Cohort 14 1 × Sham/1 × Blast Y-maze (3 days) Social recog (3 days) Trace fear cond test (1 week) Nodes of Ranvier staining (1 week) CNP staining (1 week) |
| Cohort 15 1 × Sham/1 × Blast Y-maze (8 weeks) Social recog (8 weeks) Trace fear cond test (8 weeks) Nodes of Ranvier staining (8 weeks) CNP staining (8 weeks) |
| Cohorts 16 and 17 4 × Sham/4 × Blast Y-maze (3 days) Social recog (3 days) Trace fear cond test (1 week) Nodes of Ranvier staining (1 week) CNP staining (1 week) EM analysis (1 week) for some animals |
| Cohort 18 4 × Sham/4 × Blast Social recog (8 weeks) Trace fear cond test (8 weeks) CNP staining (8 weeks) |
| Cohort 19 1 × Sham/1 × Blast Trace fear cond test (3 days) |
| Cohort 20 1 and 3 1 × Sham/1 × Blast MBP staining and Black Gold II (1 week) |
| Cohort 21 2 and 4 1 × Sham/1 × Blast MBP staining and Black Gold II (2 weeks) |
Some cohorts were used for multiple behavioral assays and biochemical or histological assays. Time shown in parentheses indicates the timing of the behavior tests performed or the animals euthanized for later histological or biochemical assays.
CNP, 2′,3-cyclic nucleotide-3-phosphodiesterase; EM, electron microscopy; MBP, myelin basic protein; RT-qPCR, reverse-transcription quantitative real-time polymerase chain reaction.