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. 2021 May 17;11:9668. doi: 10.1038/s41598-021-88414-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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¹⁸F-FDG accumulation in hypoxic cells and host-derived cells. (a) Micro-autoradiography, (b) Merged image for pimonidazole (green) and CD31 (red), and (c) merged image for pimonidazole (green), CD31 (red), and grains (magenta) in the same view of the A431 cell-derived tumor section. (d) Micro-autoradiography, (e) Merged image for vimentin (green) and CD31 (red), and (f) Merged image for vimentin (green), CD31 (red), and grains (magenta) in the same view. (g) Grain density ratio of the pimonidazole-positive region to the CD31-positive region and the vimentin-positive region to the CD31-positive region (n = 3, mean ± SEM, Student’s t-test, two-sided, *: p < 0.05). Bars: 100 μm.