Skip to main content
. 2021 May 17;28(7):1205–1220.e7. doi: 10.1016/j.stem.2021.04.028

Figure 5.

Figure 5

Presumptive corneal cell clusters from SEAM whole-eye colonies express ACE2 and TMPRSS2

(A) Relative expression of ACE2 in corneal clusters from SEAM colonies presented as UMAP and violin plot.

(B) ACE2-positive cells evaluated by Jensen TISSUES, Mouse Gene Atlas, and Gene Ontology analyses.

(C) Relative expression of TMPRSS2 in corneal clusters from SEAM colonies presented as UMAP and violin plot.

(D) TMPRSS2-positive cells evaluated by Jensen TISSUES, Mouse Gene Atlas, and Gene Ontology analyses.

(E) Table showing total cell number and percentage of the corneal cells from SEAM colonies expressing potential SARS-CoV-2 targets.

(F) Bar graph illustrating the percentage of ACE2, TMPRSS2, and ACE2+TMPRSS2-positive cells quantified by immunofluorescence (IF) staining of zone 3 in SEAM colonies (SEAM differentiated for 31 days). Error bars indicate standard error of mean (SEM) from six images counted, with an average total number of cells of per image of 916.67 ± 91.68 (mean ± SEM).

(G) Immunohistochemistry of antibodies targeting ACE2 and TMPRSS2 in SEAM colonies. White arrows indicate double-positive cells. Blue is DAPI staining illustrating nuclei in merged image, and ACE2 and TMPRSS2 are shown in green and red, respectively. Scale bars, 50 μm.