Figure 7.

Genes downregulated in limbus cells after infection with Sars-CoV-2

(A) Venn diagram of genes downregulated after infection with SARS-CoV-2 in primary adult limbus cells (brown) and limbus cell cluster from scRNA-seq of infected SEAM (purple).

(B) Log₂ fold change of overlapping downregulated genes after infection with SARS-CoV-2 at 1 MOI for 24 h in limbus cells isolated from human cadaver donors (bulk sequencing, DEseq2) and limbus clusters from SEAM organoid (scRNA-seq, Wilcoxon p < 0.05).

(C) Volcano plot showing log₂ fold change of gene expression after infection on the basis of Wilcoxon test between limbus clusters (infected cluster and nearest neighbor cluster). The inset shows the full volcano plot. Genes with log₂ fold change < −1 were labeled.

(D) Gene expression analysis on overlapping downregulated genes from BioPlanet2019, GO Biological Processes 2017b, COVID-19 Regulated Gene Sets and MSigDB Hallmark 2020. All databases accessed through Enrichr.