Table 2.

Sequence of primers for RT-PCR and long PCR.

Target gene	Primer sequence	Size (bp)	Tm (°C)
Mfn1	Forward: 5′-GGGAAGACCAAATCGACAGA-3′	152	57
	Reverse: 5′-CAAAACAGACAGGCGACAAA-3′		57
Mfn2	Forward: 5′-GAGAGGCGATTTGAGGAGTG-3′	165	58
	Reverse: 5′-CTCTTCCCGCATTTCAAGAC-3′		56
Drp1	Forward: 5′-GCCCGTGGATGATAAAAGTG-3′	215	56
	Reverse: 5′-TGGCGGTCAAGATGTCAATA-3′		56
PGC-1α	Forward: 5′-GGACGAATACCGCAGAGAGT-3′	201	59
	Reverse: 5′-CCATCATCCCGCAGATTTAC-3′		56
Nrf1	Forward: 5′-AAACCGAACACATGGCTACC-3′	168	58
	Reverse: 5′-CTGCCGTGGAGTTGAGTATG-3′		58
Tfam	Forward: 5′-TCACCTCAAGGGAAATTGAAG-3′	241	55
	Reverse: 5′-CCCAATCCCAATGACAACTC-3′		56
Long fragment	Forward:5′-AAAATCCCCGCAAACAATGACCACCC-3′	13,400	72
	Reverse: 5′-GGCAATTAAGAGTGGGATGGAGCCAA-3′		72
Shrot fragment	Forward: 5′-CCTCCCATTCATTATCGCCGCCCTGC-3′	235	60
	Reverse: 5′-GTCTGGGTCTCCTAGTAGGTCTGGGAA-3′		60
GAPDH	Forward: 5′- AGGTCGGTGTGAACGGATTTG−3′	20	58
	Reverse: 5′- GGGGTCGTTGATGGCAACA-3′		58