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. 2021 May 3;9:653207. doi: 10.3389/fcell.2021.653207

FIGURE 4.

FIGURE 4

RAB22A is a direct target of miR-204-5p. (A) Schematic illustration of RAB22A-WT and RAB22A-MuT luciferase reporter vectors and the binding sites between RAB22A and miR-204-5p. (B,C) The relative luciferase activities were detected in HuCCT1 and KMBC cells after co-transfection with RAB22A-WT/MuT and miR-204-5p mimics/NC-mimics, respectively. (D) The correlation between miR-204-5p and RAB22A in CCA tissues. (E,F) The western blot was used to detect the expression of RAB22A in HuCCT1 and KMBC cells transfected with miR-204-5p mimics and miR-204-5p inhibitors, respectively. (G) The levels of RAB22A in CCA and normal tissues and CCA cell lines were investigated using western blotting. (H) The proliferation of transfected CCA cells was determined by CCK-8 assay. (I) The levels of proliferation makers, Ki-67 and PCNA, were investigated using western blotting. All experiments were repeated at least three time. **P < 0.01, ***P < 0.001.