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. 2021 May 3;9:653207. doi: 10.3389/fcell.2021.653207

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Copyright © 2021 Tu, Chen, Zheng, Fang, Zhang, Kong, Yang, Qiu, Zhao, Lu, Lu and Ji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Circ_0021205 knockdown inhibits the tumorigenesis of CCA in vivo. (A) The presentative image of xenograft tumors was showed. (B) Tumor volumes were recorded every 4 days. (C) Tumor weights were recorded. (D) Relative miR-204-5p expression after down-regulation of circ_0021205 was measured using qPCR. (E) The levels of RAB22A in si-NC and so-circ_0021205 tumor tissues were investigated using western blotting. (F) The levels of proliferation makers, Ki-67 and PCNA, were investigated using western blotting. (G) The proliferation properties of si-NC and so-circ_0021205 tumor tissues were detected using immunohistochemistry staining. ***P < 0.001.