Fig. 6.

Decitabine-induced Treg cells ameliorate arthritis. (A–C) Mice received Treg cells intravenously 10 d after immunization with mBSA (mean ± SEM; N = 6). Knee swelling was monitored for 5 d (A). Cathepsin activity was measured using a fluorescent probe and detected by IVIS on day 2 of arthritis (B) and cell numbers of spleen, lymph node, and knee were counted on day 6 of arthritis (C). (D and E) Cells from arthritic joints were stained with antibodies against CD4⁺ T cell cytokines and lineage-specific transcription factors in D and the nTreg cell marker Helios in E. Total numbers of cells are shown in SI Appendix, Fig. S5. (F) Expression of FoxP3 and CD25 of Treg cells gated from D was quantified based on MFI. *P < 0.05, **P < 0.01, ***P < 0.001.