FIGURE 1.

Construction of engineered macrophage membrane‐enveloped nanoparticles encapsulating miR199a‐3p for the amelioration of myocardial infarction. (a) Schematic representation of engineered macrophage membrane‐enveloped nanoparticles encapsulating miR199a‐3p. Murine macrophages were transfected with plasmids encoding IL‐1βR, IL‐6R, and TNF‐αR. MiR199a‐3p was encapsulated into polyethylene glycol–polylactic acid (PEG–PLA) via a double emulsion method, denoted as NP_miR199a‐3p. Engineered macrophage membrane enveloped NPmiR199a‐3p was denoted as MMNP_miR199a‐3p. The hydrodynamic size (b) and zeta potential (c) of NP_miR199a‐3p and MMNP_miR199a‐3p were examined dynamic light scattering (DLS). (d) Representative images of NP_miR199a‐3p and MMNP_miR199a‐3p examined with transmission electron microscopy (TEM). Samples were stained with uranyl acetate. Scale bar, 100 nm