Fig. 4.

Relative abundance of the 20 most abundant bacterial families of S. pistillata determined by cDNA-based 16S rRNA gene sequencing during (A) short-term and (B) long-term heat stress. ASV counts were aggregated into bacterial families. Only the most abundant 20 families are shown; less abundant families were aggregated into the “Others” category. Upper panels represent the bacterial community at T1 and lower panels at T2. Each bar represents one of the five coral genotypes. Twelve samples are missing at T2 in the long-term heat stress experiment due to insufficient RNA for cDNA generation for bacterial community analysis.