Fig. 3.
Gating currents of monomeric D160N Hv1 mutant were ΔpH dependent. Currents produced in patches of membranes expressing the monomeric D160N Hv1 mutant at different pHs were measured. A -P/8 subtraction protocol from a subholding potential of −90 mV was applied. Representative recordings at (A) ΔpH = 0, (B) ΔpH = 1, and (C) ΔpH = 2 are shown. (D) Mean Q-V curves at the indicated pHin/pHex. Curves were fitted using a two-state Boltzmann distribution model (lines; Eq. 4 and SI Appendix, Table S2). (E) Mean ON-gating current decay time constant as a function of voltage at the indicated pHin/pHex. (Eq. 3 in text). Data are shown as mean ± SEM.