Fig. 5.

LdtF is a glycine-specific carboxypeptidase. Soluble muropeptides generated from WT cells grown in minimal A medium (36) supplemented with 50 mM glycine (A), soluble muropeptides of WT/P-ldtD (B), purified tetra (gly⁴) (C), tri-tetra (gly⁴) (D), or tetra-tetra (gly⁴) (E) were incubated either with buffer or LdtF (4 μM) and processed as described above. LdtF cleaved the terminal glycine residue completely from peak “a” (tetra-gly⁴), “b” (tri-tetra-gly⁴), or “d” (tetra-tetra-gly⁴) to yield peak 1 (tri), “c” (tri-tri), or Y (tetra-tri).