Fig 3.

S100A6 mediates the apoptosis of chondrocytes. (A) Caspase‐3 activity assays showed the value was increased in the OA model. The caspase‐3 activity was decreased after the transfection of AdS100A6 and increased after the transfection of AdsiS100A6. The effects trigged by adenovirus transfection could be offset by using LY294002 and IGF‐1, respectively. (B) TUNEL assays exhibited the similar changes to caspase‐3 activity, which were quantified by the apoptosis index. (C) The TUNEL stain images showed the nuclei were labeled with blue fluorescence, and TUNEL positive cells were labeled with red fluorescence simultaneously (× 200 magnification). Results are presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001.