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. 2021 May 1;40(10):e106214. doi: 10.15252/embj.2020106214

Figure EV5. Recovery of HIF‐1α levels in BNIP3‐silenced cells restores melanoma glycolytic phenotype and growth in vitro (related to Fig 7).

Figure EV5

  • A, B
    Murine (A) and human (B) HIF1Α transcript levels measured by qPCR in B16‐F10 cell lysates collected 48 h after plating (n = 5). They were analyzed against shCntl Luc (A) or shCntl HIF‐1α‐AA (B) using a one‐sample t‐test except human HIF1A in shBNIP3 Luc that was analyzed with Wilcoxon rank test.
  • C
    Immunoblot detection of PHD2 and ACTIN levels from B16‐F10 cell lysates collected 48 h after plating (n = 4). Densitometric quantifications are shown below each corresponding band they were analyzed using a one‐sample t‐test against shCntl Luc.
  • D
    Basal respiration rates of B16‐F10 cells assayed in galactose (25 mM galactose, 2 mM glutamine) medium using the Seahorse technology (n = 4).
  • E
    Clonogenic growth of B16‐F10 cells 10 days after seeding.

Data information: All quantitative data are mean ± SEM. Transcript expression is represented as the fold change relative to their corresponding shCntl value. *P < 0.05 **P < 0.01 when compared against shCntl Luc and $$$ P < 0.001, $$$$P < 0.0001 when compared against shCntl HIF‐1α‐AA.