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. 2021 Apr 12;40(10):e104847. doi: 10.15252/embj.2020104847

Figure EV4. Analysis of Sir2 role in repair pathway choice when the template is within unsilenced region.

Figure EV4

  1. Schematic of recombination assay on chromosome V, where a break is induced at URA3 and is repaired with a partial URA3 region (“RA”) fragment inserted on the other arm of the chromosome V.
  2. Percentage of different repair products (left) and viability (right) of WT, rad52‐R70A, and sir2Δ cells (mean ± SD; n = 3).
  3. Representative of Southern blots (left) and graph showing kinetics of BIR in H‐0 assay (right) of WT and sir2Δ cells (mean ± SD; n = 3).

Data information: Welch’s unpaired t‐test was used to determine the P‐value in all panels.