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. 2021 May 12;218(7):e20202484. doi: 10.1084/jem.20202484

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© 2021 Li et al.

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Figure S3. — Risperidone inhibits PVH non-MC4R neurons independently of MC4R. (A) Bright-field, fluorescent illumination (TRITC [tetramethylrhodamine isothiocyanate] for Mc4r-T2A-Cre::tdTomato; FITC for Alexa Fluor 488) and the merged image for whole-cell patch-clamp experiments. Arrows indicate the targeted cell. Arrowheads indicate MC4R-positive cells. Scale bars are 10 µm. (B) Acute effects of RISP (10 µM) on PVH non-MC4R neurons. (C) Acute effects of RISP in the presence of JKC-363 (20 nM). (D) Bar graphs summarize the acute effects of RISP. The dashed line in B and C indicates resting membrane potential. Data are presented as mean ± SEM. Unpaired t test. All data were verified in at least two independent experiments.