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. 2021 May 3;11:658151. doi: 10.3389/fonc.2021.658151

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Copyright © 2021 Shih, Chuang, Tsai, Chen, Chuang, Lu and Lai

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MALAT1 is located primarily in the cytoplasm of MCF-7 and MDA-MB-231 cells under normoxia or hypoxia. (A–D) Cytoplasmic and nuclear distribution of MALAT1 in breast cancer cells [MCF-7 (A), MDA-MB-231 (B)] and lung cancer cells [A549 (C), H1299 (D)] cells under normoxia. (E, F) Cytoplasmic and nuclear distribution of MALAT1 in MCF-7 (E) and MDA-MB-231 (F) cells under hypoxia. Relative abundance of RNA was normalized to the total amount of RNA and detected by qPCR. BCAR4 and U6 snRNA: nuclear marker. GAPDH: cytoplasmic marker. Data shown are the means ± SDs (n=3). (G) RNA FISH of MALAT1. Cell nuclei were stained by Hoechst staining (blue). MALAT1 was hybridized with MALAT1-FITC probes (green) in breast cancer cell lines and detected by a Zeiss LSM880 microscope. Magnification:1,000×; Scale bar: 5 μm.