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. 2021 May 3;11:658151. doi: 10.3389/fonc.2021.658151

Figure 5.

Figure 5

MALAT1 binds to miR-3064-5p directly. (A, B) RIP using antibody against AGO2. The relative RNA levels of MALAT1 (A) and miR-3064-5p (B) were quantified and normalized to the IgG group using qPCR. Input: positive control; IgG: negative control. (C) Schematic representation of firefly reporter constructs containing the fragments of Site 1 (1,279~1,302 bp) and Site 2 (7,837~7,860 bp) of MALAT1, and mutants with mutation at the binding sites of miR-3064-5p. (D, E) Luciferase reporter assays of MALAT1 fragment with wild-type or mutant (m) Site 1 (D) or Site 2 (E) in cells overexpressing miR-3064-5p mimic. HEK-293T cells were transfected with miR-3064-5p mimic and firefly/Renilla plasmids. The relative firefly luciferase activity was measured and normalized to Renilla luciferase activity. Data shown are the means ± SDs (n=3). *P < 0.05.