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. 2021 May 3;11:658151. doi: 10.3389/fonc.2021.658151

Figure 6.

Figure 6

Knockdown of MALAT1 decreases proliferation and metastasis in MDA-MB-231 cells. (A) Measurement of cell proliferation using MTT assays. Cell growth was measured in MDA-MB-231 cells transduced with lentivirus which expresses shRNA against MALAT1. The proliferation rate was normalized to day zero. (B) Wound healing assay. Left: Representative pictures of wound healing assays. After 24 h of transduction, wound healing was photographed at 0, 12, and 24 h. Right: Migration ability was quantified as reduction in wound size at 24 h. (C) Colony formation assay. Top: Representative pictures of colony formation assays. Colonies with cell numbers more than fifty were counted. Bottom: Quantification of results. (D) Cell cycle distribution by flow cytometry. Top: Representative diagrams of flow cytometry. After 48 h of transfection, cells were harvested and stained with PI. Bottom: Quantification of results as the percentage of cells in each phase of the cell cycle. All data shown are the means ± SDs (n = 3). *P < 0.05.