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. 2021 May 3;11:658151. doi: 10.3389/fonc.2021.658151

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Copyright © 2021 Shih, Chuang, Tsai, Chen, Chuang, Lu and Lai

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of MALAT1 promotes proliferation and migration in MCF-7 cells. (A) Measurement of cell proliferation using MTT assays. Cell growth was measured in MCF-7 cells overexpressing MALAT1. The proliferation rate was normalized to day zero. (B) Wound healing assay. Left: Representative pictures of wound healing assays. After 24 h of transfection, wound healing was photographed at 0, 12, and 24 h. Right: Migration ability was quantified as reduction in wound size at 24 h. (C) Colony formation assay. Top: Representative pictures of colony formation assays. Colonies with cell numbers more than fifty were counted. Bottom: Quantification of results. (D) Cell cycle distribution by flow cytometry. Top: Representative diagrams of flow cytometry. After 48 h of transfection, cells were harvested and stained with PI. Bottom: Quantification of results as the percentage of cells in each phase of the cell cycle. (E) A proposed model for illustrating the regulatory mechanisms and function of hypoxia-induced lncRNA MALAT1 in breast cancer cells. All data shown are the means ± SDs (n = 3). *P < 0.05.