Table 1.
The primers for reverse transcription and quantitative RT-PCR.
| Gene/miRNA | Primer | Sequence (5' to 3') |
|---|---|---|
| Reverse Transcription | ||
| miR-378c | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACCCACTC | |
| miR-3064-5p | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACTTGCAC | |
| miR-3150a-3p | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACCCAACC | |
| miR-4325 | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACTCACTG | |
| miR-7855-5p | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACCCGAGC | |
| U6 snRNA | CGCTTCACGAATTTGCGTGTCAT | |
| Quantitative RT-PCR | ||
| MALAT1 | Forward | GACGGAGGTTGAGATGAAGC |
| Reverse | ATTCGGGGCTCTGTAGTCCT | |
| BCAR4 | Forward | GTTCCGATGCTTGTCTTGCTC |
| Reverse | CCAAAGACGAAGATGCCAGG | |
| U6 snRNA | Forward | GCTTCGGCAGCACATATACTAAAAT |
| Reverse | CGCTTCACGAATTTGCGTGTCAT | |
| GAPDH | Forward | AACGGGAAGCTTGTCATCAATGGAAA |
| Reverse | GCATCAGCAGAGGGGGCAGAG | |
| 18s rRNA | Forward | TCAACTTTCGATGGTAGTCGCCGT |
| Reverse | TCCTTGGATGTGGTAGCCGTTTCT | |
| miR-378c | Forward | GCGGCGGACTGGACTTGGAGTCAGAA |
| Reverse | GTGCAGGGTCCGAGGT | |
| miR-3064-5p | Forward | GCGGCGGTCTGGCTGTTGTGGT |
| Reverse | GTGCAGGGTCCGAGGT | |
| miR-3150a-3p | Forward | GCGGCGGCTGGGGAGATCCTCGA |
| Reverse | GTGCAGGGTCCGAGGT | |
| miR-4325 | Forward | GCGGCGGTTGCACTTGTCT |
| Reverse | GTGCAGGGTCCGAGGT | |
| miR-7855-5p | Forward | GCGGCGGTTGGTGAGGACCCCAA |
| Reverse | GTGCAGGGTCCGAGGT | |