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Effect of sample storage over one month under different conditions on SARS-CoV-2 RNA concentrations (N1 gene target, gc/ml wastewater). Error bars represent standard deviations of replicate samples. Samples stored as non-processed raw wastewater (WW) at 4 °C or −20 °C exhibited lower concentrations compared to samples stored at -20 °C as concentrate (post ultrafiltration) or RNA extracts. Indices a and b denote experimental conditions yielding statistically different sample means.
