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. 2021 Apr 27;24(1):485. doi: 10.3892/mmr.2021.12124

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Copyright: © Kang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — NTS inhibits TLR-4 and IL-6 mRNA expression. (A) Reverse transcription-semi-quantitative PCR results showing the analysis of TLR-4 and IL-6 mRNA expression following the treatment of C2C12 cells with 100 ng/ml LPS or 0.2 and 0.5 µg/ml NTS for 24 h. (B) Relative mRNA expression levels of TLR-4 and IL-6 were determined via densitometry and were normalized to GAPDH mRNA. Data are representative of three independent experiments. **P<0.01 and ***P<0.001 vs. control. NTS, non-toxic sulfur; TLR-4, Toll-like receptor 4; LPS, lipopolysaccharide; Con, control.