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. 2019 May 10;101(2):347–359. doi: 10.1093/biolre/ioz089

Figure 1.

Figure 1.

SPO11 structure and CRISPR-Cas9 editing strategy. (A) Schematic of human SPO11 protein with known domains and location of the P306T targeted alteration. Stars indicate known metal binding sites. (B) Organization of human SPO11. The SPO11 P306T variant encoded by SNP rs185545661 is located in exon 11. The red nucleotides are in the codon encoding P306 and T306 and indicate the nucleotide (C) changed by homologous recombination. Blue nucleotides indicate a designed silent HaeIII restriction enzyme site added to the mouse allele via editing of T to the underlined C, and the underlined CCC indicates the PAM site. Italicized sequence corresponds to guide sequence. (C) Sanger sequencing chromatograms of WT and Spo11P306T/P306T mice, with the relevant codon bases shaded blue.