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. 2019 May 10;101(2):347–359. doi: 10.1093/biolre/ioz089

Figure 5.

Figure 5.

Spo11P306T/P306T oocytes have delayed DSB formation but die from unrepaired DSBs in late Prophase I. (A) Representative P0 oocyte chromosome spreads immunolabeled with RAD51 and SYCP3. Size bar = 25μm. “MUT” refers to Spo11P306T/P306T. (B) Quantification of RAD51 foci. Late zygonema: WT (n = 2, cells = 18, avg = 36 ± 4.3) vs mutant (n = 2, cells = 37, avg = 23 ± 3.7); pachynema: WT (n = 2, cells = 24, avg = 5.5 ± 1.1) vs mutant (w = 2, cells = 70, avg = 10 ± 1.5); diplonema: WT (n = 2, cells = 20, avg = 0.9 ± 0.3) vs mutant (n = 2, cells = 48, avg = 3 ± 0.7). Avg ± SEM. (C) Quantification of primordial follicles in 3 week old females, with or without an intact DNA damage checkpoint. The leftmost 3 datasets and the lightmost dataset are identical to those in Figure 2F. Each data point is one ovary, and both ovaries were counted from each female. Statistics done by Student's t-test.